The Interplay between Atherosclerosis and Cancer: Breast Cancer Cells Increase the Expression of Endothelial Cell Adhesion Markers

Cardiovascular diseases are the leading causes of death worldwide, closely followed by cancer. To investigate impact breast cancer cell lines (SKBR3, MCF-7, and MDA-MB-231) on endothelial adhesion, a blended medium containing 30% breast-cancer-conditioned was prepared. This then exposed to human umbilical vein cells (HUVECs) monocytes (THP-1) for 48 h. Homemade oxidized low-density lipoproteins (oxLDL) were optionally added medium. Immunofluorescence performed assess expression E-selectin, connexin-43, ICAM-1 HUVECs, as well LOX-1, CD36, CD162 THP-1. Additionally, unoxidized LDL three h, formation oxLDL quantified. Our results revealed an upregulation all six adhesion markers involved in initiation atherosclerosis when HUVECs THP-1 Furthermore, this further increased exposure oxLDL. We also observed significant elevation levels cells. In conclusion, our findings successfully demonstrate oxidation presence cells, accompanied augmented receptors initiation. These shed new light clinically interplay between